BIOCHEMISTRY AND SEPARATION TECHNIQUES


Theoretical Course

TIME
TITLE
SHORT CONTENTS
1- 2
Traditional analytical methods
Protein separation by solubility (pH, salt concentration, inorganic solvent). Dialysis, electrodialysis, ultrafiltration, membrane filtration. Liophylisation.
3-4
Centrifugation
Centrifuge types, rotor types. Sedimentation. Differential pelleting, Zonal centrifugation, Ultracentrifugation.
5-6
Chromatography I.
Gas Chromatography (GC), Liquid Chromatography (LC), Paperchromatography, Thin Layer Chromatography (TLC), Columnchromatography, High Performance Liquid Chromatography (HPLC), Fast Protein Liquid Chromatography (FPLC).
7-8
Chromatography II.
Gel Chromatography, Ion Exchange Chromatography, Chromatofocusing, Affinity Chromatography, Reverse Phase Chromatography.
9-10
Electrophoresis I.
Paper Electrophoresis, Thin Layer Electrophoresis, Agarose Gel Electrophoresis, Native Polyacrylamide Gel Electrophoresis, SDS Polyacrylamide Gel Electrophoresis, Electrofocusing.
11-12
Electrophoresis II.
Immuno Electrophoresis (IEF), Capillar Electrophoresis.
13-14
Immunoanalytical methods
Radio Immuno Assay (RIA), Immuno Radiometric Analysis ( IRMA), Enzyme-linked Immunosorbent Assay (ELISA), Blotting techniques: western blot, southern blot.



Practical Course

TITLE
SHORT CONTENTS
Separation of Serum Protein
  • Determination of Serum Protein Concentration
  • Separation of Serum Protein by Ion Exchange Chromatography
  • SDS Gel Electrophoresis of Fractions
Kinetic Analysis of Trypsin
  • Determination Km and Vmax of Trypsin by BAPNA
  • Inhibition of Trypsin by Soja Trypsininhibitor